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Tools and Reagents

We continuously develop new tools and reagents, which we are happy to share.

Bio-miRTa

MicroRNAs are known to play context-dependent roles, and as such, their functionally important gene targets can vary in distinct pathological conditions. Therefore, we developed an algorithm, Bio-miRTa, for the identification of biologically relevant miRNA targets, which enables the ranking of the miRNA candidate target genes according to the biological process of interest.


Download R Package


If you use this tool or data, please cite the following publication: 

Iacovos P. Michael, Sadegh Saghafinia, and Douglas Hanahan. A set of microRNAs coordinately controls tumorigenesis, invasion, and metastasis. Proc National Acad Sci 116, 24184–24195 (2019).

Protocols

  • Neuroendocrine Patient-derived Organoids (contact us)
  • Neuroendocrine Patient-derived Xenograft ex-ovo models (contact us)

Reagents

Cell lines

Cell lines

Cell lines

  • 99-3o; male mouse PanNET IT-like cell line (PMID: 31063757)
  • AJ-52571-1; male mouse PanNET MLP-like cell line (PMID: 31063757)

Plasmids

Cell lines

Cell lines

The Michael Lab is an avid user of the piggyBac transposon system and has generated various transposons for inducible gene gain- and loss-of-function and inducible miRNA expression. 

Complete list of plasmids and maps

Copyright © 2023 Michael Lab  

Tumor Biology and Precision Oncology


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